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Polymeric particles for targeted drug delivery into mononuclear phagocytic system
Prošková, Denisa ; Holas, Ondřej (advisor) ; Šnejdrová, Eva (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Technology Supervisor: PharmDr. Ondřej Holas, Ph.D. Consultant: Dmytro Iefremenko, M.Sc. Student: Denisa Prošková Title of Thesis: Polymeric paticles for targeted drug delivery into mononuclear fagocytic system Polymeric nanoparticles (NPs) can act as drug nanocarriers. They are frequently used to enhance distribution to a target tissues in which they specifically act. The mononuclear phagocytic system (MPS) represents a target to which NPs could be specifically distributed. It has been found that cells of this system are involved in the development of a number of diseases. Optimal parameters of NPs suitable for MPS targeting are size ranging between 100 nm and 300 nm and negative surface charge. In this work the polymeric nanoparticles with encapsulated cholic acid were prepared by nanoprecipitation method using five selected types of non-ionic surfactants in concentration of 0,1%, 0,5%, 1% and 2%. PLGA was the polymer used for their preparation because of its high biocompatibility and biodegradability. Encapsulation efficiency, size and zeta-potential were selected as critical parameters chosen for prepared nanoparticles. The obtained data were used to characterize the effect of non-ionic surfactants on the...
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Polymeric particles for targeted and enhanced bioavailability
Kozáčková, Daniela ; Holas, Ondřej (advisor) ; Svačinová, Petra (referee)
7 ABSTRACT Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of: Pharmaceutical Technology Mentor: PharmDr. Ondřej Holas, Ph.D. Consultant: Mgr. Barbora Boltnarová Student: Daniela Kozáčková Title of thesis: Polymeric particles for targeted and enhanced bioavailability Nanoparticles prepared from biodegradable and biocompatible polymers are useful for targeted therapy of inflammatory diseases and increase the bioavailability of poorly water-soluble anti-inflammatory drugs. Targeted distribution is mainly mediated by the physico-chemical properties of nanoparticles. This can reduce unwanted side effects of encapsulated drug. The ideal properties of nanoparticles for passive targeting to cells of the mononuclear phagocytic system (MPS) are size in the range of 100 nm to 300 nm, hydrophobic character and negative surface charge. Matrix-type polymeric nanoparticles were prepared. Prepared nanoparticles were evaluated for size, polydispersity index, zetapotential and encapsulation efficiency of the drug. Three anti-inflammatory substances with different water solubility - a dexamethasone, a dexamethasone acetate and a curcumin were encapsulated. The matrix of nanoparticles was made of PLGA copolymer, three types of PLGA with different lactide:glycolide ratio were used. Nanoparticles...
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Polymeric particles for targeted drug delivery into mononuclear phagocytic system
Prošková, Denisa ; Holas, Ondřej (advisor) ; Šnejdrová, Eva (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Technology Supervisor: PharmDr. Ondřej Holas, Ph.D. Consultant: Dmytro Iefremenko, M.Sc. Student: Denisa Prošková Title of Thesis: Polymeric paticles for targeted drug delivery into mononuclear fagocytic system Polymeric nanoparticles (NPs) can act as drug nanocarriers. They are frequently used to enhance distribution to a target tissues in which they specifically act. The mononuclear phagocytic system (MPS) represents a target to which NPs could be specifically distributed. It has been found that cells of this system are involved in the development of a number of diseases. Optimal parameters of NPs suitable for MPS targeting are size ranging between 100 nm and 300 nm and negative surface charge. In this work the polymeric nanoparticles with encapsulated cholic acid were prepared by nanoprecipitation method using five selected types of non-ionic surfactants in concentration of 0,1%, 0,5%, 1% and 2%. PLGA was the polymer used for their preparation because of its high biocompatibility and biodegradability. Encapsulation efficiency, size and zeta-potential were selected as critical parameters chosen for prepared nanoparticles. The obtained data were used to characterize the effect of non-ionic surfactants on the...
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Polymeric particles for targeted anti-inflammatory therapy
Santarius, Karolina ; Holas, Ondřej (advisor) ; Smékalová, Monika (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Technology Mentor: PharmDr. Ondřej Holas, PhD. Consultant: Mgr. Barbora Boltnarová Student: Karolina Santarius Title of thesis: Polymeric particles for targeted anti-inflammatory therapy Macrophages are heterogenous population of cells, that play crucial role in inflammatory diseases like NASH. It was found that liver macrophages polarization into phenotype M1 is major factor in NASH progression. It seems that induced conversion from proinflammatory macrophages into anti-inflammatory is one of the potencial therapeutic target. This macrophages polarization can be achieved by drug delivery system based on drug-loaded nanoparticles. The aim of this work was to prepare and optimize the preparation of nanoparticles formulation based on polymeric nanoparticles loaded dexamethasone as model substance. The nanoparticles formulation was prepared by nanoprecipitation method using three PLGA polymers with different lactic/glycolic acid ratios. Several surfactants in various concentrations were used to optimize preparation. Prepared dexamethasone-loaded nanoparticles were characterized in terms of their size and polydisperzity index using dynamic light scattering. Zeta potencial of nanoparticles was determine by...
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Polymeric particles for targeted and enhanced bioavailability
Kozáčková, Daniela ; Holas, Ondřej (advisor) ; Svačinová, Petra (referee)
7 ABSTRACT Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of: Pharmaceutical Technology Mentor: PharmDr. Ondřej Holas, Ph.D. Consultant: Mgr. Barbora Boltnarová Student: Daniela Kozáčková Title of thesis: Polymeric particles for targeted and enhanced bioavailability Nanoparticles prepared from biodegradable and biocompatible polymers are useful for targeted therapy of inflammatory diseases and increase the bioavailability of poorly water-soluble anti-inflammatory drugs. Targeted distribution is mainly mediated by the physico-chemical properties of nanoparticles. This can reduce unwanted side effects of encapsulated drug. The ideal properties of nanoparticles for passive targeting to cells of the mononuclear phagocytic system (MPS) are size in the range of 100 nm to 300 nm, hydrophobic character and negative surface charge. Matrix-type polymeric nanoparticles were prepared. Prepared nanoparticles were evaluated for size, polydispersity index, zetapotential and encapsulation efficiency of the drug. Three anti-inflammatory substances with different water solubility - a dexamethasone, a dexamethasone acetate and a curcumin were encapsulated. The matrix of nanoparticles was made of PLGA copolymer, three types of PLGA with different lactide:glycolide ratio were used. Nanoparticles...
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